Calocybella pudica (Bon & Contu) Vizzini, Consiglio & Setti, in Vizzini, Consiglio, Setti & Ercole, IMA Fungus 6(1): 5 (2015)
Pileus 5–25 mm diam, hemisphericalcampanulate, then plano-convex, occasionally umbonate, non-hygrophanous, dry, smooth, orange to brownish-orange, sometimes paler over the margin. Lamellae medium crowded, adnatesmarginate, lamellulae l = (0) 1–2 (3), bright yellow or ochraceous yellow; edge paler. Stipe 25–60 × 2–8 mm, cylindrical, sometimes tapered at the base, firm, pale yellow or brownish yellow, finely white-pruinose, fibrillose-striate; base subtended by yellow rhizoids. Context firm, elastic, yellowish, more or less rapidly changing to red on bruising or when exposed and to violaceous-red after applying a drop of 10 % NH3 or 5 % KOH; odour mealy, taste mealy then slightly astringent.
Basidiospores [163, 5, 5] (3.8–) 4.9–6.0 (–6.7) × (2.8–) 3.0– 3.7 (4.3) μm, Q = (1.3–) 1.46–1.80 (–2.1), Qm = 1.63 ± 0.17, V = (16.7–) 23.7–40.6 (–61.4) μm3, Vm = 32.2 ± 8.4 μm3, ellipsoid to elongate-ellipsoid to oblong, abaxially or adaxially often flattened, even sub-lacrymoid in front view, hilar appendix rather long and prominent, content granular or mono- to multi-guttulate, inamyloid, cyanophilous, smooth to verrucose. Basidia 25–33 × 6.9–7.9 μm, fourspored, rarely two-spored, clavate, occasionally with preapical or central constriction, siderophilous (with internal siderophilous/cyanophilous granules); sterigmata up to 6 μm long. Hymenophoral trama regular to subregular, made up of hyphae 3–10 μm wide, becoming red in L4 and yellow in Melzer’s. Subhymenium constituted by short, septate elements, 2–4 μm wide. Cheilocystidia none. It was observed the occurrence of numerous misshapen basidioles or basidia, exhibiting apical extroflexions (to 20 × 1.5–2 μm), constrictions, or the upper part may be swollen or subcapitate. Pleurocystidia none. Pileipellis: suprapellis a cutis of subparallel, variously interwoven hyphae, 2–8 μm wide, with slightly gelatinized outermost layer, yellow in Melzer’s, smooth, cylindrical, with smooth, undifferentiated to slightly enlarged terminal elements, up to 6 μm wide; pigment intracellular, of more or less dark yellow colour, some with a very fine epiparietal pigment; subpellis consisting of elongate hyphae, 4–12 μm wide; trama hyphae cylindrical, to 12 μm wide. Stipe hyphae cylindrical, 8–14 μm wide within the stipe; cortical hyphae 2.5–5 μm wide, smooth, with smooth, undifferentiated to slightly enlarged, round-tipped terminal elements. Clamp connections present throughout.
Habitat and distribution: Gregarious, in small groups, usually fasciculate, in grassy clearings with Juncus sp.; so far known from Italy, France, and Spain.
Index Fungorum number: IF811740
Figure 1. Bayesian phylogenetic analysis based on the LSU sequences of Lyophyllaceae, with Entoloma sinuatum and E. prunuloides as outgroup taxa. BPP values (in bold) ≥ 0.7 and MLB values ≥ 50 % are shown on the branches. Thickened branches indicate Bayesian posterior probability > 0.95 and ML bootstrap support > 70 %. For each sequenced taxon the Genbank/UNITE number is given. Newly sequenced collections are in bold.
Figure 2. Bayesian phylogenetic analysis based on selected ITS sequences of Lyophyllaceae, with Ossicaulis lachnopus and O. lignatilis as outgroup taxa. BPP values (in bold) ≥ 0.7 and MLB values ≥ 50 % are shown on the branches. Thickened branches indicate Bayesian posterior probability > 0.95 and ML bootstrap support > 70 %. For each sequenced taxon the Genbank/UNITE number is given. Newly sequenced collections are in bold.