Agaricales » Lyophyllaceae » Clitolyophyllum

Clitolyophyllum akcaabatense

Clitolyophyllum akcaabatense Sesli, Vizzini & Contu, in Sesli et al., Canadian Journal of Botany 94(2): 75 (2016)

Etymology: “akcaabatense” comes from “Akcaabat”, one of the districts of Trabzon.

Diagnosis: It is distinguished by fruiting on the bark of Picea orientalis; fan shaped and umbilicate, thin and striated pileus, whitish and decurrent lamellae; 2–4 spored basidia; smooth and ellipsoid spores (5.5–) 6.0– 8.0(–9) m × (3.3–)3.5–5(–6.0) m; and unique ITS/LSU/ rpb2 sequences.

Basidiomata clitocyboid. Hyphal system monomitic. Pileus 30–55(–60) mm broad, fan shaped, with a slightly costate to undulating, folded and irregular margin with a small depression resembling a navel and a V-shaped slit where it is connected to the stipe; surface smooth, dull, slightly hygrophanous, translucent-striate toward to margin, gray-beige to beige-brownish, horn-gray, or wood colour when moist (Pale Ochraceous-Buff, Ochraceous- Buff, Yellow Ocher, Cinnamon-Buff, Chamois, Avellaneous), slightly darker toward the centre (Orange-Cinnamon,; Isabella ColorWood Browm,), palecream colored (Light Buff) when dry. Lamellae decurrent, broad towards pileus’ centre, shallow towards margin, thin, L = 35–45, I = 1–4, interveined at base, at first whitish then light cream or beige (Pale Ochraceous- Salmon, Pale Ochraceous-Buff). Stipe 20– 30 mm × 4–7 mm, laterally attached to the pileus, cylindrical to flattened, hollow with enlarged apex and tapering towards the base, sometimes twisted, tough, elastic, at apex smooth and concolorous with pileus surface, 2/3 of the surface towards the base covered with a typical white to creamy, woolly mycelium. Context thin, white to gray-beige. Odour fungoid. Taste indistinct. Spore-print white.

Basidiospores (5.5–)6.0–8.0(–9) μm × (3.3–)3.5–5(–6.0) μm, on average 7.2 μm × 4.2 μm (n = 100), E = 1.50–1.85, Q = 1.58–1.78, ellipsoid, ellipsoid–fusoid, sublacrymoid, thin-walled, hyaline, smooth, nondextrinoid, cyanophilic. Basidia 25–30(–37) μm × 6.5–7.5 μm, 2–4 spored, clavate or subcylindrical, without siderophilous granulation, with basal clamp connection. Basidioles 15–20 μm × 3.5– 5.0 μm, narrowly clavate, subfusoid, subcylindrical. Hymenial cystidia not observed. Hymenophoral trama regular, consisting of cylindrical, subinflated, thin- to slightly thick-walled, nondextrinoid parallel hyphae 3.5– 60(–90) μm × (7.5–)15–25 μm. Pileipellis made up of irregularly cylindrical, clavate, thin- to slightly thickwalled, smooth, subinflated hyphae 40–50(–100) μm × (5.5–)20–30 μm; some hyphae slightly gelatinized with intracellular pigment; terminal elements adpressed to suberect, sometimes pileocystidia-like. Stipitipellis a cutis of cylindrical, parallel, slightly thick-walled, smooth, nondextrinoid, hyphae 5.5–15 μm wide. Caulocystidia 35–45 μm × 4.5–15 μm, irregular, cylindrical, subfusoid or almost subulate, sometimes branched, thin- to slightly thick-walled. Clamp-connections present in all tissues.

 

Habit, Habitat, And Distribution: solitary to gregarious, growing on the dead bark of Picea orientalis together with mosses. Fruiting in autumn, so far known only from Turkey.

Index Fungorum number: IF814483

 

Figure. 1. Phylogeny of selected members of the Tricholomatoid clade based on a Bayesian and Maximum Likelihood Inference analysis of a supermatrix of three nuclear gene regions (nrITS, nrLSU, and rpb2). Bayesian posterior probability (BPP) values (in bold) ≥0.75 and Maximum Likelihood bootstrap (MLB) values ≥50% are shown on the branches. The newly sequenced collection is in bold.

Figure. 2. Clitolyophyllum akcaabatense gen. nov., sp. nov. (a–c) Basidiomata in situ (holotype). Scale bars = 10 mm. Photos by E. Sesli.

 

Figure. 3. Clitolyophyllum akcaabatense gen. nov., sp. nov. Microscopic features in ammoniacal Congo red (holotype). (a–b) Lamellar cross sections. (a) General appearance. (b) Hyphae of the hymenophoral trama, basidia and basidiospores. (c) Basidia and basidioles. Scale bars: a–b = 20 m; c = 10 m. Photos by E. Sesli.

Figure. 4. Clitolyophyllum akcaabatense gen. nov., sp. nov. Microscopic features (holotype). (a) Elements of the pileipellis. (b) Aerial hyphae on the stipe. (c–f) Basidiospores: (c) in Melzer’s reagent; (d) in ammoniacal Congo red; (e) in water; (f) germinating spores in ammoniacal Congo red. Scale bars: a–b = 20 m; c–f = 10 m. Photos by E. Sesli.

 

Reference:

Sesli, E., Vizzini, A. Contu, M. 2016. Clitolyophyllum akcaabatensis gen. nov., sp. nov. (Agaricales, Tricholomatineaea), a new fan shaped clitocybioid agaric from Turkey. Canadian Journal of Botany. 94:73-80

 

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