Holtermanniales » Rhynchogastremaceae » Bandoniozyma

Bandoniozyma noutii

Bandoniozyma noutii Boekhout, Fell, Scorzetti & Theelen 2012, in Valente et al., PLoS ONE 7(10): e46060 (2012).

Etymology: The specific epithet noutii refers to Robert (Rob) Nout, investigator of traditionally fermented foods from South America, Africa and Asia.

Diagnosis: After growth for 7 days in 2% glucose medium at 25^oC, a sediment and film are formed. Cells are ovoid, subglobose to globose, 4–6×4–5 µm, usually with polar budding but also with multilateral budding, with buds that may adhere into short chains (Figure 2a). Pseudohyphae are present and fall apart in filaments that measure 28–50(-70)×2–4 µm, and eventually become somewhat thick-walled, irregularly broadened or somewhat broadened on one side. After 5 days on 5% malt extract agar at 25^oC, colonies are 10 mm in diameter, convex, grayish cream-beige, shiny, strongly mucoid, smooth, and with an entire margin that may form sectors. Cells measure 3.3–7.8×3–7 µm. Under a cover glass filaments and hyphae occur with cells measuring 8–40×2–2.5 µm. On Dalmau plate on yeast morphology agar, pseudohyphae occur with cells measuring 8–40×2–2.5 µm. Sexual reproduction is absent. Mixing the three available strains on potato dextrose agar, oat meal agar, corn meal agar, malt extract agar, glucose-yeast extract agar, and yeast extract malt extract agar did not show any indication of a mating reaction.

Index Fungrum Number: IF563852

 

Figure 1. ITS tree showing the phylogenetic relationship among the Tremellomycetes, with emphasis on the Bandoniozyma clade, obtained by neighbor-joining analysis using Mega 5.0. Most species belong to the order Tremellales, with the exception of Cystofilobasidium capitatum (Cystofilobasidiales), Cryptococcus albidus (Filobasidiales), Trichosporum cutaneum (Trichosporonales), and the outgroup Farysizyma itapuensis (Ustilaginales). The numbers given on the branches are the frequencies with which a given branch appeared in 10,000 bootstrap replications. Bar, substitutions per nucleotide position.

 

Figure 2. Cell morphologies: a) Bandoniozyma noutii CBS 8364T after 7 days in YM broth at 256C. Bar = 10 mm, b) Bandoniozyma tunnelae CBS 8024T after 7 days in YM broth at 256C. Bar = 10 mm, c) Bandoniozyma fermentans CBS 12399T grown in YMA for 3 day at 25uC. Bar = 10 mm, d) Bandoniozyma glucofermentans CBS 10381T after 7 days in YM broth at 256C. Bar = 10 mm, e) Bandoniozyma aquatica CBS 12527T grown in YEPD broth for 3 days at 256C. Bar = 10 mm, and f) Bandoniozyma complexa CBS 11570T grown in YEPD broth for 3 days at 256C. Bar = 10 mm.

 

Reference:

Valente, P., Boekhout, T., Landell, M. F., Crestani, J., Pagnocca, F. C., Sette, L. D., Passarini, M. R. Z., Rosa, C. A., Brandão, L. R., Pimenta, R. S., Ribeiro, J. R., Garcia, K. M., Lee, C. F., Suh, S. O., Péter, G., Dlauchy, D., Fell, J. W., Scorzetti, G., Theelen, B., & Vainstein, M. H. (2012). Bandoniozyma gen. nov., a Genus of Fermentative and Non-Fermentative Tremellaceous Yeast Species. PLoS ONE, 7(10), e46060.

 

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