Chernovia houtui

Chernovia houtui J. Federici, A.M. Yurkov & D. Begerow 2016, in Yurkov et al., Mycological Progress, 15, 845-859 (2016).

Etymology: the epithet “houtui” derives from the Chinese mythological figure of Houtu, a deity of the earth and soil.

Diagnosis: After growth on YM agar plates for 7 days at 16 and 22 °C, the streak culture is cream to yellowish-cream, mucoid with a smooth and glistening surface. The margin is entire. Colony is getting tan and wrinkled after 2 months of incubation. After growth on YM agar plates for 7 days at 16 and 22 °C, cells are subglobose to ovoid (3–4 × 4–7 μm), occurring singly or in pairs, and proliferating by polar budding (Figure 2h). Pseudohyphae and true hyphae were not observed after 1 month in Dalmau plate culture on CMA at 16–22 °C. Maximum growth temperature: 30 °C.

Assimilation of carbon compounds: growth on D-glucose, D-galactose, D-xylose, L-arabinose, sucrose, maltose, a,a-trehalose, cellobiose, raffinose, melezitose, D-glucitol (delayed), D-mannitol, D-glucoronate, DL-lactate, succinate, citrate, 4- hydroxybenzoic acid and 3,4-dihydroxybenzoic acid. Growth

reaction in form of a ring occurred on ethanol. Weak growth occurs on D-ribose, lactose, glycerol, ribitol, myo-inositol and ferulic acid. No growth occurs on L-sorbose, D-glucosamine, D-arabinose, L-rhamnose, inulin, starch, D-saccharic acid, malic acid, L-tartaric acid, vanillic acid and trans-ferulic acid. Assimilation of nitrogen compounds: nitrate, nitrite, L-lysine and D-tryptophan.

Molecular characteristics (type strain): nucleotide sequences of LSU (D1/D2 domains) rRNA deposited in NCBI/EMBL (GenBank) under the accession number LT548267.

Index Fungorum Number: IF816159

Figure 1. Maximum likelihood analysis of an alignment of the SSU rRNA and the LSU (D1/D2 domains) rRNA showing phylogenetic relationships of Wallemiomycetes and other families comprising the phylum Basidiomycota. The analysis is based on the dataset TreeBase S13396 (Nguyen et al. 2013). Branches corresponding to classes other than Wallemiomycetes and Tremellomycetes are collapsed. The numbers on branches are frequencies (>50 %) with which a given branch appeared in 100 bootstrap replications. The scale bars indicate the numbers of expected substitutions accumulated per site